02977nas a2200289   4500008004100000020001400041245017000055210006900225260001200294300001600306490000700322520198600329653001402315653001702329653001802346653003002364653001802394653002402412653003502436653002202471100003202493700003602525700002402561700001902585700002202604856006102626       2018                        eng d  a1365-269900aThe interplay of riverscape features and exotic introgression on the genetic structure of the Mexican golden trout (Oncorhynchus chrysogaster), a simulation approach0 ainterplay of riverscape features and exotic introgression on the  c2018///  a1500 - 15140 v453 aAim We explored the factors between riverscape features and exotic introgression of cultured rainbow trout (Oncorhynchus mykiss) that drive the current genetic structure in the endemic Mexican golden trout (Oncorhynchus chrysogaster) using a riverscape genetics simulator. Location Sierra Madre Occidental, Northwest Mexico, North America. Methods We created a system model for Mexican golden trout to test various scenarios for the effect of riverscape resistance and rainbow trout introgression on the native genetic structure of the Mexican golden trout. Individual genotypes were used to initialize the model and were based upon empirical allele frequencies from three genetically and spatially distinct groups in the study area. The geographical location of the simulated populations was defined using a species distribution model. We included hypotheses for movement of riverine distance and riverscape resistance. We evaluated model performance by conducting population and landscape genetics analyses on the simulation outputs. Results The most plausible scenario included riverscape resistance without introgression, which presented a spatial genetic structure matching the observed riverscape genetic structure and fitting expected general life-history patterns for high elevation trout streams. In addition, temperature, precipitation, hydrology and topography were the most important variables influencing species occurrence and dispersal. Main conclusion This work presents one of the first empirically derived simulation studies of introgression. It highlights the importance of considering riverscape resistance (i.e. hydroclimatic and topographical gradient effects) in models of spatial genetic structure and introgression. Moreover, our modelling suggests that what we characterize as riverscape resistance is acting as a boundary against exotic introgression. The results of this study could help to develop management strategies that preserve native gene pools.10aCDMetaPOP10aexotic trout10aMexican trout10aOncorhynchus chrysogaster10arainbow trout10ariverscape genetics10aspecies distribution modelling10aTruchas Mexicanas1 aEscalante, Marco, Alejandro1 aDe León, Francisco, J. García1 aRuiz‐Luna, Arturo1 aLandguth, Erin1 aManel, Stéphanie  uhttp://onlinelibrary.wiley.com/doi/abs/10.1111/jbi.1324602599nas a2200181   4500008004100000020001400041245021700055210006900272260001200341520189700353100002502250700002102275700003602296700002202332700002702354700002202381856001402403       2020                        eng d  a1566-062100aGenotyping-by-sequencing reveals the effects of riverscape, climate and interspecific introgression on the genetic diversity and local adaptation of the endangered Mexican golden trout (Oncorhynchus chrysogaster)0 aGenotypingbysequencing reveals the effects of riverscape climate  c2020///3 aHow environmental and anthropogenic factors influence genetic variation and local adaptation is a central issue in evolutionary biology. The Mexican golden trout (Oncorhynchus chrysogaster), one of the southernmost native salmonid species in the world, is susceptible to climate change, habitat perturbations and the competition and hybridization with exotic rainbow trout (O. mykiss). The present study aimed for the first time to use genotyping-by-sequencing to explore the effect of genetic hybridization with O. mykiss and of riverscape and climatic variables on the genetic variation among O. chrysogaster populations. Genotyping-by-sequencing (GBS) was applied to generate 9767 single nucleotide polymorphisms (SNPs), genotyping 272 O. chrysogaster and O. mykiss. Population genomics analyses were combined with landscape ecology approaches into a riverine context (riverscape genetics). The clustering analyses detected seven different genetic groups (six for O. chrysogater and one for aquaculture O. mykiss) and a small amount of admixture between aquaculture and native trout with only two native genetic clusters showing exotic introgression. Latitude and precipitation of the driest month had a significant negative effect on genetic diversity and evidence of isolation by river resistance was detected, suggesting that the landscape heterogeneity was preventing trout dispersal, both for native and exotic individuals. Moreover, several outlier SNPs were identified as potentially implicated in local adaptation to local hydroclimatic variables. Overall, this study suggests that O. chrysogater may require conservation planning given (i) exotic introgression from O. mykiss locally threatening O. chrysogater genetic integrity, and (ii) putative local adaptation but low genetic diversity and hence probably reduced evolutionary potential especially in a climate change context.1 aEscalante, Marco, A.1 aPerrier, Charles1 aDe León, Francisco, J. García1 aRuiz-Luna, Arturo1 aOrtega-Abboud, Enrique1 aManel, Stéphanie  u10/gg6rgj