Truchas Mexicanas

studying and working toward conservation of Mexico's diverse native trout

Isolation and characterization of 17 tetranucleotide microsatellite loci in the Mexican golden trout (Oncorhynchus chrysogaster, Needham & Gard 1964) derived from 454 pyrosequencing

Publication Type:Journal Article
Year of Publication:2013
Authors:García-De-León, FJ, O. Lozano-Garza, A, Mayden, RL, Delgado-Vega, R, Camarena-Rosales, F, Ruiz-Campos, G, Escalante-Sánchez, MA, Ruiz-Luna, A, Del Río-Portilla, MÁngel, Barriga-Sosa, Ide los A, Arcos-Ortega, GFabiola, Paniagua-Chávez, C
Journal:Conservation Genetics Resources
Pagination:1009 - 1012
Date Published:2013/05/14/
ISBN Number:1877-7252, 1877-7260
Keywords:Mexican golden trout, Mexican trout, Oncorhynchus chrysogaster, Sierra Madre Occidental, Truchas Mexicanas

We present seventeen microsatellite loci developed for the Mexican golden trout Oncorhynchus chrysogaster through GS-FLX 454 pyrosequencing. We selected 34 primer pairs tested on 19 trout specimens from The Rio Verde (Rio Fuerte Bain) and we tested cross amplification on 35 trout specimens collected in seven hydrological basins from the Sierra Madre Occidental and Sierra San Pedro Mártir, as well as five hatchery reared Oncorhynchus mykiss. We observed medium and high genetic variation among loci (mean number of alleles per locus = 8.0, SD = 2.92; mean H O = 0.65, SD = 0.27). These loci are the first to be characterized explicitly for O. chrysogaster and they are expected to be useful for population studies in this species and as reference for species delimitation.

Short Title:Conservation Genet Resour
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith