02000nas a2200169   4500008004100000245016400041210006900205260001600274520134000290100002401630700002101654700003301675700002301708700002801731700002201759856004901781       2018                        eng d00aEffect of riverscape and exotic introgression on the genetic structure and local adaptation of the endangered Mexican golden trout (                          )0 aEffect of riverscape and exotic introgression on the genetic str  c2018/12/19/3 aHow environmental and anthropogenic factors influence genetic variation and local adaptation is a central issue in evolutionary biology. The Mexican golden trout (            
            ), one of the southernmost native salmonid species in the world, is susceptible to climate change, habitat perturbations and the introduction of exotic rainbow trout (
            
            ) for aquaculture. The present study aimed to explore the effect of both genetic introgression from
            
            and riverscape variables on the genetic variation of
            
            . Genotyping-by-sequencing (GBS) was applied to generate 9 767 single nucleotide polymorphisms (SNPs), genotyping 270
            
            and aquaculture trout. Population genomics analyses were combined with riverscape genetics approaches. A small degree of admixture was identified between aquaculture and native trout, and significant effects of riverscape variables on genetic diversity were detected, as well as evidence of isolation by riverscape resistance within a basin. Gene-environment associations identified genes potentially implicated in adaptation to local hydroclimatic variables. This is an integrative study using a non-common multi-scale approach in landscape genetics for the conservation of an endangered riverine species.
1 aEscalante, Marco, A1 aPerrier, Charles1 ade Leon, Francisco, J Garcia1 aLuna, Arturo, Ruiz1 aAbboud, Enrique, Ortega1 aManel, Stéphanie  uhttp://biorxiv.org/lookup/doi/10.1101/50004102599nas a2200181   4500008004100000020001400041245021700055210006900272260001200341520189700353100002502250700002102275700003602296700002202332700002702354700002202381856001402403       2020                        eng d  a1566-062100aGenotyping-by-sequencing reveals the effects of riverscape, climate and interspecific introgression on the genetic diversity and local adaptation of the endangered Mexican golden trout (Oncorhynchus chrysogaster)0 aGenotypingbysequencing reveals the effects of riverscape climate  c2020///3 aHow environmental and anthropogenic factors influence genetic variation and local adaptation is a central issue in evolutionary biology. The Mexican golden trout (Oncorhynchus chrysogaster), one of the southernmost native salmonid species in the world, is susceptible to climate change, habitat perturbations and the competition and hybridization with exotic rainbow trout (O. mykiss). The present study aimed for the first time to use genotyping-by-sequencing to explore the effect of genetic hybridization with O. mykiss and of riverscape and climatic variables on the genetic variation among O. chrysogaster populations. Genotyping-by-sequencing (GBS) was applied to generate 9767 single nucleotide polymorphisms (SNPs), genotyping 272 O. chrysogaster and O. mykiss. Population genomics analyses were combined with landscape ecology approaches into a riverine context (riverscape genetics). The clustering analyses detected seven different genetic groups (six for O. chrysogater and one for aquaculture O. mykiss) and a small amount of admixture between aquaculture and native trout with only two native genetic clusters showing exotic introgression. Latitude and precipitation of the driest month had a significant negative effect on genetic diversity and evidence of isolation by river resistance was detected, suggesting that the landscape heterogeneity was preventing trout dispersal, both for native and exotic individuals. Moreover, several outlier SNPs were identified as potentially implicated in local adaptation to local hydroclimatic variables. Overall, this study suggests that O. chrysogater may require conservation planning given (i) exotic introgression from O. mykiss locally threatening O. chrysogater genetic integrity, and (ii) putative local adaptation but low genetic diversity and hence probably reduced evolutionary potential especially in a climate change context.1 aEscalante, Marco, A.1 aPerrier, Charles1 aDe León, Francisco, J. García1 aRuiz-Luna, Arturo1 aOrtega-Abboud, Enrique1 aManel, Stéphanie  u10/gg6rgj